ICSI Center

LABORATORY OF ADVANCED REPRODUCTIVE BIOLOGY AND GENETICS

We are dedicated to implementing cutting-edge research in the field of Reproductive Biology. Our primary emphasis lies in exploring the complexities of human gametes–spermatozoa and oocytes–to gain insights into their maturation processes and interactions. Beyond that, our investigation extends to understanding the genetic underpinnings of infertility, employing advanced CRISPR-Cas9 editing techniques.

A significant hallmark of our laboratory is our pioneering success in devising and implementing a groundbreaking gender selection method for spermatozoa prior to Intrauterine Insemination (IUI) and Intracytoplasmic Sperm Injection (ICSI).

American Society for Reproductive Medicine Annual Meeting 2018

Differential Expression of Testicular Function Can Reveal Why Some NOA Men Successfully Yield Spermatozoa

Philip Xie, Stephanie Cheung, Claire O’Neill, Alessandra Parrella, Mohamed Irani, Zev Rosenwaks, and Gianpiero D. Palermo

Claire germ cell ASRM2018.jpg

Microfluidic Selection of Spermatozoa Retains
Chromatin Integrity and Yields Higher Pregnancy Rates

Alessandra Parrella, Philip Xie, Derek Keating, Zev Rosenwaks, and Gianpiero D. Palermo

Microfluidics SCF poster SC.jpg

Feasibility of Gene Knockout in Human Sperm Cells Using CRISPR-Cas9

Diane Choi, Alessandra Parrella, Brandon Chin, Claire O’Neill, Stephanie Cheung, Zev Rosenwaks, and Gianpiero D. Palermo

Crispr ASRM2018 SC.jpg

Differentiating Mouse Induced Pluripotent Stem Cells into Male Germ Cells Through Embryoid Bodies

Vivian Lu, Alessandra Parrella, Stephen Chow, Claire O’Neill, Mohamed Irani, Spencer Connaughty, Aysha Trout, Zev Rosenwaks, and Gianpiero D. Palermo

ASRM EB Poster SC.jpg

RNA Sequencing to Assess Embryonic Developmental Competence of the Male Gamete

Alessandra Parrella, Claire O’Neill, Zev Rosenwaks, and Gianpiero D. Palermo

RNA GENE EXPRESSION SC.jpg

Genomic Profiling of Azoospermic Men Provides Information
on Their Gametes’ Ability to Support Embryogenesis

Stephanie Cheung, Zev Rosenwaks, and Gianpiero D. Palermo

Fertile Infertile NOA OA genes p472.jpg

Sperm Chromatin Fragmentation Has No Discernable Effect on ICSI Embryo Ploidy

Derek Keating, Alessandra Parrella, Mohamad Irani, Zev Rosenwaks, and Gianpiero D. Palermo

asrmposter embryo ploidy SC.jpg

Understanding the Role of Cytoplasmic Dysmaturity on ICSI Outcome

Alessandra Parrella, Derek Keating, Mohamad Irani, Zev Rosenwaks, and Gianpiero D Palermo

oocyte poster SC.jpg

Microfluidic Selection of Spermatozoa
Retains Chromatin Integrity and Yields Higher Pregnancy Rates

Alessandra Parrella, Philip Xie, Derek Keating, Zev Rosenwaks, and Gianpiero D. Palermo

Microfluidics and PGD SC.jpg

In Vitro Neospermatogenesis of Human Induced Pluripotent Stem Cells

Mohamad Irani, Alessandra Parrella, Claire O’Neill, Vivian Lu, Diane Choi, Zev Rosenwaks, and Gianpiero D. Palermo

hiPSC ASRM 2018 SC.jpg

DNA Sequencing of the Sperm Genome Retrieved at Different Levels of the Male Genital Tract

S. Cheung, G. D. Palermo, Z. Rosenwaks

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Interpreting the Spermatogenic Profile of NOA Men That Fail to Yield Spermatozoa

C. O’Neill, A. Parrella, M. Irani, Z. Rosenwaks, G. D. Palermo

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In Vitro Culture of Neonatal Mouse Testicular Cells Maintained on Decellularized Seminiferous Tubule

P. Xie, Z. Rosenwaks, G. D. Palermo

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